Human mitochondrial DNA with large deletions repopulates organelles faster than full-length genomes under relaxed copy number control.

Assessment of mitochondrial DNA copy number in peripheral blood leukocyte of opiate abusers and healthy individuals

Background: Based on the studies, variation in the mitochondrial DNA (mtDNA) copy number in peripheral blood leukocytes is associated with increased susceptibility to diseases including cancer. Opiate abusers are at high risk for diseases. In this study, we measured the mtDNA copy number in peripheral blood leukocytes in a group of opiate abusers compared with those in healthy individuals. Met...

O-9: The Central Role of Mitochondrial Function in Quality of Human Oocyte

Background: Mitochondria are the most aboudent and small essential organelles found in eukaryotic cells. These are semiautonomous organelles for the production of cellular ATP that through its various biochemical pathways. The primary pathway for ATP production is OXPHOS via the electron transfer chain (ETC) which is encoded by nuclear DNA and mtdna genomes. Mitochondria consist of double stran...

Mechanisms of human mitochondrial DNA maintenance: the determining role of primary sequence and length over function.

Although the regulation of mitochondrial DNA (mtDNA) copy number is performed by nuclear-coded factors, very little is known about the mechanisms controlling this process. We attempted to introduce nonhuman ape mtDNA into human cells harboring either no mtDNA or mutated mtDNAs (partial deletion and tRNA gene point mutation). Unexpectedly, only cells containing no mtDNA could be repopulated with...

Simultaneous quantification of mitochondrial DNA copy number and deletion ratio: A multiplex real-time PCR assay

Mitochondrial dysfunction is implicated in a vast array of diseases and conditions, such as Alzheimer's disease, cancer, and aging. Alterations in mitochondrial DNA (mtDNA) may provide insight into the processes that either initiate or propagate this dysfunction. Here, we describe a unique multiplex assay which simultaneously provides assessments of mtDNA copy number and the proportion of genom...

A Novel Large-Scale Deletion of The Mitochondrial DNA of Spermatozoa of Men in North Iran